The current stage of human development is characterised by an increasing demand for environmentally friendly plant raw materials for use in the pharmaceutical industry. However, the uncontrolled harvesting of medicinal plant species leads to the depletion of their natural reserves and a decline in population sizes, which may ultimately result in the extinction of these species. Therefore, it is crucial to identify alternative sources for obtaining such plant materials. Among the valuable rare plant species in need of protection within the territory of Ukraine are species of the genus Carlina L., in particular, Carlina onopordifolia Besser ex Szafer, Kulcz. et Pawl and Carlina cirsioides Klokov. Both are listed in the Red Data Book of Ukraine (2009) and are classified as vulnerable species. The range of Carlina acaulis L., which is considered regionally rare in Ukraine, is also rapidly declining.